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BENSON'S MICROBIOLOGY APPLICATION: LABORATORY MANUAL IN GENERAL MICROBIOLOGY 10e(rb)2007
Author: ALFRED E. BROWN
ISBN: 0071254161
Year: 2007
Publisher: MCGRAW-HILL EDUCATION ASIA
Category: MICROBIOLOGY
Edition: 10
Format: Ring Binding
Language: English
Pages: 544
 ABOUT THE TITLE
The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained clearly illustrated exercises and full-color format make Microbiological Applications: Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course this manual assumes no prior organic chemistry course has been taken.
Features
• Content has been updated throughout especially in Part 8: Identification of Unknown Bacteria and in Part 13: Medical Microbiology and Immunology to include the latest research. Also updated was Exercise 78: A Synthetic Epidemic in keeping with lab safety requirements.
• Benson s Complete Version (78 experiments) provides the largest variety of laboratory exercises to accommodate all laboratory curricula--from a medical emphasis to an environmental emphasis and everything between. (A Short Version with 60 experiments is also available.)
• Full-color examples of lab test results appear in the lab exercises so students have an immediate reference against which they can compare their results.
• Because you may prefer or require a different way to stage a lab exercise this lab manual includes suggestions for alternative approaches and procedures in the appendixes and in the Instructor s Manual.
• The Instructor s Manual provides a materials list and guidelines for conducting all the experiments as well as answers to the lab exercises. It can be found at www.mhhe.com/labcentral
• Customize this book through Primis Online! This title is part of the Primis Online Database: www.mhhe.com/primis/online. You can customize this book to meet your exact needs and mix and match with other items on Primis Online--allowing you maximum choice and flexibility. Instructors can also choose between two delivery formats: custom printed books (in black and white) or custom eBooks (in color).
AUTHOR:
Alfred E Brown
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 TABLE OF CONTENTS
Benson's Microbiological Applications, Laboratory Manual in General Microbiology, Complete Version, 10th Edition
Part 1 Microscopy
Exercise 1 Brightfield Microscopy
Exercise 2 Darkfield Microscopy
Exercise 3 Phase-Contrast Microscopy
Exercise 4 Fluorescence Microscopy
Exercise 5 Microscopic Measurements
Part 2 Survey of Microorganisms
Exercise 6 Protozoans, Algae, and Cyanobacteria
Exercise 7 Ubiquity of Bacteria
Exercise 8 The Fungi: Yeasts and Molds
Part 3 Manipulation of Microorganisms
Exercise 9 Aseptic Technique
Exercise 10 Pure Culture Techniques
Part 4 Staining and Observation of Microorganisms
Exercise 11 Smear Preparation
Exercise 12 Simple Staining
Exercise 13 Negative Staining
Exercise 14 Capsular Staining
Exercise 15 Gram Staining
Exercise 16 Spore Staining: Two Methods
Exercise 17 Acid-Fast Staining: Ziehl-Neelsen Method
Exercise 18 Motility Determination
Part 5 Culture Methods
Exercise 19 Culture Media Preparation
Exercise 20 Preparation of Stock Cultures
Exercise 21 Cultivation of Anaerobes
Exercise 22 Enumeration of Bacteria: The Standard Plate Count
Exercise 23 Slime Mold Culture
Exercise 24 Slide Culture: Molds
Part 6 Bacterial Viruses
Exercise 25 Determination of a Bacteriophage Titer
Exercise 26 Burst Size Determination: A One-Step Growth Curve
Exercise 27 Isolation of Phage from Flies
Exercise 28 Phage Typing
Part 7 Enviornmental Influences and Control of Microbial Growth
Exercise 29 Temperature: Effects on Growth
Exercise 30 Temperature: Lethal Effects
Exercise 31 pH and Microbial Growth
Exercise 32 Water Activity and Osmotic Pressure
Exercise 33 Ultraviolet Light: Lethal Effects
Exercise 34 Oligodynamic Action
Exercise 35 Evaluation of Alcohol: Its Effectiveness As an Antiseptic
Exercise 36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
Exercise 37 Evaluation of Antiseptics: The Filter Paper Disk Method
Exercise 38 Effectiveness of Hand Scrubbing
Part 8 Identification of Unknown Bacteria
Exercise 39 Morphological Study of Unknown Bacterium
Exercise 40 Cultural Characteristics
Exercise 41 Physiological Characteristics: Oxidation and Fermentation Tests
Exercise 42 Physiological Characteristics: Hydrolytic Reactions
Exercise 43 Physiological Characteristics: Biochemical Tests
Exercise 44 Use of Bergey's Manual
Part 9 Miniaturized Multitest Systems
Exercise 45 Enterobacteriaceae Identification: The API 20E System
Exercise 46 Enterobacteriaceae Identification: The Enterotube II System
Exercise 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System
Exercise 48 Staphyococcus Identification: The API Staph-Ident System
Part 10 Diversity and Environmental Microbiology
Exercise 49 Isolation of an Antibiotic Producer: The Actinomyces
Exercise 50 Nitrogen Cycle: Ammonification
Exercise 51 Symbiotic Nitrogen Fixation: Rhizobium
Exercise 52 Free-Living Nitrogen Fixation: Azotobacter
Exercise 53 Denitrification: Paracoccus Denitrificans
Exercise 54 The Winogradsky Column
Exercise 55 Purple Nonsulfer Photosynthetic Bacteria
Exercise 56 Sulfate Reducing Bacteria: Desulfovibrio
Exercise 57 Bacterial Commensalism
Exercise 58 Bacterial Synergism
Exercise 59 Microbial Antagonism
Part 11, Applied Microbiology
Exercise 60 Bacterial Counts of Foods
Exercise 61 Bacteriological Examination of Water: Qualitative Tests
Exercise 62 The Membrane Filter Method
Exercise 63 Reductase Test
Exercise 64 Microbial Spoilage of Canned Food
Exercise 65 Microbiology of Alcohol Fermentation
Part 12 Bacterial Genetics and Biotechnology
Exercise 66 Mutant Isolation by Replica Plating
Exercise 67 Bacterial Transformation
Exercise 68 Polymerase Chain Reaction for Amplifying DNA
Exercise 69 Plasmid Isolation
Part 13 Medical Microbiology and Immunology
Exercise 70 The Staphylococci: Isolation and Identification
Exercise 71 The Streptococci: Isolation and Identification
Exercise 72 Gram-Negative Intestinal Pathogens
Exercise 73 Slide Agglutination Test: Serological Typing
Exercise 74 Slide Agglutination (Latex) Test: For S. aureus Identification
Exercise 75 Tube Agglutination Test: The Heterophile Antibody Test
Exercise 76 White Blood Cell Study: The Differential WBC Count
Exercise 77 Blood Grouping
Exercise 78 A Synthetic Epidemic
Appendix A: Tables
Appendix B: Indicators, Stains, Reagents
Appendix C: Media
Appendix D: Identification Charts
Appendix E: The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics
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